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eclipse ti-e epifluorescence live imaging microscope  (Nikon)

 
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    Nikon eclipse ti-e epifluorescence live imaging microscope
    Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E <t>epifluorescence</t> <t>microscope</t> (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.
    Eclipse Ti E Epifluorescence Live Imaging Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eclipse ti-e epifluorescence live imaging microscope/product/Nikon
    Average 90 stars, based on 1 article reviews
    eclipse ti-e epifluorescence live imaging microscope - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "A Computational Approach to Identify Novel Protein Targets Uncovers New Potential Mechanisms of Action of Mirtazapine S (+) and R (−) Enantiomers in Rett Syndrome"

    Article Title: A Computational Approach to Identify Novel Protein Targets Uncovers New Potential Mechanisms of Action of Mirtazapine S (+) and R (−) Enantiomers in Rett Syndrome

    Journal: Journal of Neurochemistry

    doi: 10.1111/jnc.70093

    Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E epifluorescence microscope (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.
    Figure Legend Snippet: Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E epifluorescence microscope (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.

    Techniques Used: In Vitro, In Silico, Drug discovery, Immunofluorescence, Microscopy, Software, Control, Concentration Assay, Two Tailed Test, Comparison



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    Nikon eclipse ti-e epifluorescence live imaging microscope
    Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E <t>epifluorescence</t> <t>microscope</t> (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.
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    Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E <t>epifluorescence</t> <t>microscope</t> (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.
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    Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E epifluorescence microscope (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.

    Journal: Journal of Neurochemistry

    Article Title: A Computational Approach to Identify Novel Protein Targets Uncovers New Potential Mechanisms of Action of Mirtazapine S (+) and R (−) Enantiomers in Rett Syndrome

    doi: 10.1111/jnc.70093

    Figure Lengend Snippet: Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E epifluorescence microscope (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.

    Article Snippet: Fluorescent images were captured using a Nikon Eclipse Ti‐E epifluorescence live imaging microscope, equipped with a Nikon DS‐Qi2 camera (CMOS sensor, 16.25 megapixel), and a 10X objective lens.

    Techniques: In Vitro, In Silico, Drug discovery, Immunofluorescence, Microscopy, Software, Control, Concentration Assay, Two Tailed Test, Comparison